Common Applications

Below is a list of techniques commonly used within our Flow Core:

  • Immunophenotyping
  • DNA/RNA: cell cycle, aneuploidy, endoreduplication, kinetics
  • DNA base ratios
  • Chromatin structure
  • Apoptosis (DNA degradation, mitochondrial membrane potential, permeability changes, caspase activity)
  • Membrane potential
  • Membrane fluidity
  • Membrane fusion/runover
  • Intracellular calcium (ions) flux
  • Intracellular pH
  • Sulfhydryl groups/glutathione
  • Cell viability
  • Cell tracking and proliferation
  • Intracellular reactive oxygen species (Oxidative burst)
  • Cell proliferation
  • Cell enumeration
  • Cell volume and morphological complexity
  • Cell pigments (eg. chlorophyll or phycoerythrin)
  • Drug delivery
  • Multidrug resistance (MDR)
  • Phagocytosis
  • Pathogen-host cell adherence
  • Differentiation
  • Identification of “stem cells”
  • Reticulocyte, platelet etc analysis
  • Microparticles analysis
  • Assessing infection/transfection levels
  • Monitoring of the electropermeabilization of cells
  • Cytotoxicity assay
  • Enzymatic activity
  • Cell activation
  • Protein-protein interactions (FRET, split-GFP)
  • Protein modifications, phospho-proteins
  • Activation of signalling pathways
  • Cytokine Secretion
  • Sorting (f.ex sperm sorting for sex preselection)
  • Karyotyping
  • Telomere length
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