Intro to Flow Cytometry Course

Back by popular demand!

Register early to ensure your spot.

On Thursday, October 1 at 1pm the FoMD flow core will be hosting our Introduction to Flow Cytometry course! Whether you’re new to flow or want a refresher, come out and join us!

If interested or for more information, please email Aja Rieger at aja@ualberta.ca.

Topics covered will include:
  • Applications of flow cytometry
  • How does a flow cytometer work?
  • Sorting 101
  • Imaging and flow
  • Choosing fluorochromes and the basics of compensation
  • Controls- why do I need them and how do I choose them?
  • Quality assurance and quality control in flow cytometry
  • Data analysis principles and software

When: Thursday October 1, 1-4pm

Where: Oborowsky Degner Hall (1-040 LKS) (course will be capped at 50)

To register, please email aja@ualberta.ca with your name, lab, and level of studies (i.e. undergrad, technician, etc.).

 

Last-line-of-defense---statistics

Another panel design resource

The FoMD flow core is now subscribed to Chromocyte! If you haven’t had a chance to check out Chromocyte, you should take a look. It is an online resource for flow cytometrists designed by flow cytometrists!

On Chromocyte, you can design panels, find suppliers, products and services. They also have a fantastic ‘educate’ and ‘communicate’ sections with training resources, assays and protocols, news, tips, and a panel of experts that you can pose your questions to. I highly recommend you check out this resource!

You can also access the FoMD core configurations at:
https://www.chromocyte.com/cf/fomdflow

See the poster below for more information!

Facility Poster for – Flow Cytometry Core Service

Finally, below are PDF’s of our instrument configurations that list all possible fluorochromes that can be used:

Instrument Configuration Poster for Fortessa SORP

Instrument Configuration Poster for LSRFortessa Katz

Instrument Configuration Poster for LSRFortessa X20

Instrument Configuration Poster for Canto II

Instrument Configuration Poster for AriaIIIu

Instrument Configuration Poster for AriaIII (Hal and Contagion)

 

Some new things in the flow core

Hi all! Some new things to be aware of:

1. The Fortessa X20 is now online! Anyone looking to do live BSL2 samples, today is your day!! If you would like to use this machine, please contact Aja (aja@ualberta.ca) to arrange training for the instrument or bioBUBBLE.

2. FlowJo v10 is now available in the analysis room (HMRC 650).

For those looking for a little more training on the analysis software, check out the webinars below!
FlowJo webinars

FCS Express webinars

3. For those of you using BioLegend reagents, check out the deal posted outside the flow core! For each publication you publish using BioLegend reagents (max 4/year), you can receive $250 in products!!

 

As usual, don’t hesitate to contact me with questions, etc.

Until next time!

 

Last-line-of-defense---statistics

New online panel design software

Screen Shot 2015-04-17 at 9.24.33 AM

Looking for a software to help you design your flow cytometry panel? Well look no further!

Fluorish Panel Builder is a free software (Mac and PC compatible) that assists in panel design. It takes the pain out of searching databases to determine available antigen/fluorochrome combinations- and even selects fluorochromes that work with your instrument configuration. All configurations for the FoMD flow core units have been uploaded- so by joining our core, you can customize your panel to the unit(s) you use!

Other added features:

  • Compare and select antibodies from various companies (and can choose which companies to search)
  • Get pricing and ordering information
  • Can create a lab inventory of antibodies
  • Save and share your panels
  • Create panels for a wide range of species (mouse, human, rat, fish, frog, primate, and more!)
  • Not just for antibodies- also for all fluorescent dyes

For more information or to download your free version, check out the website today!

 

And for those of you interested in other new technologies, reagents, and software, check out Expert Cytometry’s Top Flow Cytometry Instruments, Reagents & Software To Look Forward To In 2015 (1st Annual List) 

 

logo-tonbo

Finally, to make your panel design even easier, check out Tonbo BioScience Ready-Set-Flow! Backbone Panels here today!

Intro to flow cytometry course

Save the date!!!

On Monday, May 11 at 12pm the FoMD flow core will be hosting our first Introduction to Flow Cytometry course! Whether you’re new to flow or want a refresher, come out and join us!

If interested or for more information, please email Aja Rieger at aja@ualberta.ca.

Topics covered will include:
  • Applications of flow cytometry
  • How does a flow cytometer work?
  • Sorting 101
  • Imaging and flow
  • Choosing fluorochromes and the basics of compensation
  • Controls- why do I need them and how do I choose them?
  • Quality assurance and quality control in flow cytometry
  • Data analysis principles and software

When: Monday, May 11; 12-4pm

Where: ECHA 1 498 (course will be capped at 70)

To register, please email aja@ualberta.ca with your name, lab, and level of studies (i.e. undergrad, technician, etc.).

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FCS Express 4 now available on the network drive!

In an attempt to make your analysis easier and dongle-free, we now have a network license for FCS Express v4. To access it, log in with the MED credentials and go to the K: drive (if you need information on how to map the K: network to your computer, follow the directions here: Mapping K drive. Directions are given for both Macs and PCs).

Once in the K: drive, go into the FCS Express v4 folder and right click on the FCS Express shortcut icon. Copy and paste this to your desktop. Now you are good to analyze!!

Update on the Flow Cytometry Core Facility and User Committee

Please join us in welcoming Dr. Aja Rieger as the FoMD Flow Cytometry Core Facility Manager.  Dr. Aja Rieger is an accomplished scientist, returning to us from a post-doctoral fellowship at University of California, Berkeley. She received her B.Sc. in Immunology and Infection from the University of Alberta, a M.Sc. in Microbiology and Immunology from McGill University, and a Ph.D. in Physiology, Development and Cell Biology from the University of Alberta, for which she held the Vanier Scholarship.  She has extensive experience in flow cytometry, with a specialization in imaging flow cytometry and multi-parametric experimental design.  We look forward to her contributions to building the core, in-depth training and education in flow cytometry and consulting with users in assay development and research design. She has joined us just in time to support training the immanent onslaught of the summer students!

 

We would also like to take this opportunity to thank Dr. Kevin Kane for his more than four years of service as Chair of the Flow Cytometry Core User Committee and the eight years prior while it was facility through MMI.  Through his leadership, the facility expanded significantly with respect to infrastructure and capabilities.  It now serves multiple faculties and we hope it will continue to flourish and grow.

 

On behalf of the User Committee,

 

Debby Burshtyn, Ph.D.                                                             Tom Hobman, Ph.D.

Associate Professor, MMI                                                         Professor of Cell Biology

Chair, Flow Cytometry Core User Committee                 Associate Dean Research – Facilities

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