BD FACS Aria III (BSL1)
The BD FACSAria III cell sorter is the high-speed benchtop digital flow cytometer. It is equipped with five spatially separated air-cooled lasers – 488 nm, 633 nm, 561 nm,375 nm and 405 nm. The near UV (375 nm) laser use the same path as violet (405 nm) laser 375nm and cannot be use simultaneously.
Sorting can be performed into two or four tubes, at three flow rates, with the speed of up to 35,000 events/sec . Most cell types can be accommodated with the use of 100, 85 or 70 micron nozzles. The Automated Cell Deposition Unit (ACDU) makes it possible to sort individual cells into multi-well plates, PCR tubes or microscope slides. Instrument is also equipped with Aerosol Management Option (AMO), a device that traps aerosolized particles. The system operates on a PC-based Windows XP platform using BD FACSDiva software. Instrument is located in Room 667 HMRC.
Operation of the cell sorter is performed only by the lab staff and appointment must be arranged in advance.
Filter Configuration- please note that the dyes are just representative dyes on each filter. It is better to look at the emission filter.