BD FACS Aria III (BSL2)
The BD FACSAria III cell sorter is the high-speed benchtop digital flow cytometer. It is equipped with five spatially separated air-cooled lasers – 488 nm, 633 nm, 561 nm, 375 nm and 405 nm. The near UV (375 nm) laser use the same path as violet (405 nm) laser 375nm and cannot be use simultaneously.
FACSAria III is enclosed by the BioProtect IV ClassII Type A2 biosafety cabinet from Baker Company. The cabinet provides HEPA filtration and airflow control to maximize the protection of the operators from the potentially hazardous samples.
Sorting can be performed into two or four tubes, at three flow rates, with the speed of up to 35,000 events/sec . Most cell types can be accommodated with the use of 130, 100, 85 or 70 micron nozzles. The Automated Cell Deposition Unit (ACDU) makes it possible to sort individual cells into multi-well plates, PCR tubes or microscope slides. The system operates on a PC-based Windows XP platform using BD FACSDiva software. Instrument is located in Katz 6-125.
This instrument is available for user training.